欧美另类69xxxxx,波多野吉衣 美乳人妻,波多野结av衣东京热无码专区,在教室伦流澡到高潮HGL视频

主營產(chǎn)品:

ELISA試劑盒,人ELISA試劑盒,大鼠ELISA試劑盒,小鼠ELISA試劑盒,裸鼠ELISA試劑盒,倉鼠ELISA試劑盒,標準品,培養(yǎng)基和生物試劑等

18221656311

聯(lián)系我們/CONTACT US

聯(lián)系人:錢經(jīng)理

電 話:18221656311

手 機:18221656311

地 址:上海市松江臨港科技城漢橋文化科技園B座

郵 編:200093

傳 真:021-64881400

郵 箱:2885617636@qq.com

阿儀網(wǎng)商鋪:http://www.app17.com/c58469/

手機網(wǎng)站:m.hybiosh.com

產(chǎn)品分類/CLASS

ELISA試劑盒

酶聯(lián)免疫試劑盒

人ELISA試劑盒

進口血清

抗體

標準品

Sigma試劑

Amresco試劑

食品檢測試劑盒

Spectrum試劑

免疫化學產(chǎn)品

其他方法測試盒

金標試劑盒

放免試劑盒

代理品牌

技術文章ARTICLE

恒遠生物生物素標記的山羊抗兔引用文獻

點擊次數(shù):12611   發(fā)布時間:2020/12/29 15:58:38

【文獻標題】Upregulation of microRNA-141 contributes to suppressing epithelial-mesenchymal transition and lymph node metastasis in laryngeal cancer through HOXC6-dependent TGF-βsignaling pathway

【作者】Li Chen,De-Zhong Sun,Yu-Gui Fu,et.al

【作者單位】臨沂市人民醫(yī)院(Linyi People’s Hospital)

【文獻中引用產(chǎn)品】

生物素標記的山羊抗兔

【關鍵詞】microRNA-141, TGF-β signaling pathway, Homeobox C6, epithelial-mesenchymal transition, metastasis, laryngeal cancer

【DOI】https://doi.org/10.1016/j.cellsig.2019.109444

【影響因子(IF)】6.40

出版期刊】《Cellular Signalling》

【產(chǎn)品原文引用】

ImmunohistochemistryThe positive expression rates of HOXC6 in laryngeal cancer and adjacent normal tissues were detected by immunohistochemistry. Laryngeal cancer tissues and adjacent normal tissues were fixed in 4% paraformaldehyde for 24 h, dehydrated using 80%, 90% and 100% ethanol successively, cleared with n-butanol, placed in a paraffin box at 60°C and embedded in paraffin. After that, the tissues were cut into 5 μm-thick slices, deparaffinized with xylene after being heated at 60°C for 1 h. The slices were then dehydrated by gradient alcohol and soaked in 3% H2O2 for 10 min, followed by antigen retrieval. Next, the slices were boiled in 0.01 M citric acid buffer (pH = 6.0) at 95°C for 15 - 20 min, and incubated with 100 μL 5% bovine serum albumin (BSA) blocking solution at 37°C for 30 min, followed by incubating with 100 μL primary rabbit antibody to HOXC6 (1 : 50, PB0948, Wuhan Boster Biological Technology, Ltd., Wuhan, Hubei, China) overnight at 4°C. The slices were then incubated with biotin-labeled goat anti-rabbit (1 : 100, HY90046, Shanghai HengYuan Biological Technology Co., Ltd., Shanghai, China) at 37°C for 30 min and with streptavidin-peroxidase solution (Beijing Zhongshan Biological Technology Co., Ltd., Beijing, China) at 37°C for 30 min, and then developed by diaminobenzidine staining solution (Beijing Bioss Biological Technology Co., Ltd., Beijing, China) at room temperature. Subsequently, the slices were soaked in hematoxylin for 5 min, rinsed with 1% hydrochloric acid for 4 s and washed under tap water for 20 min. Brownish-yellow cells were confirmed as HOXC6 positive cells [23]. Five high-magnification visual fields were randomly selected from each slice with 100 cells per field. HOXC6 positive expression was defined by the percentage of positive cells: number of HOXC6 positive tumor cells/number of total tumor cells > 10% was counted as positive (+), < 10% was regarded as negative (-). Experiments were repeated 3 times independently.


完整版PDF文獻請咨詢在線客服或者電話聯(lián)系我司業(yè)務員!

更多公司福利請關注“恒遠生物”微信公眾號!

原創(chuàng)作者:上海恒遠生物科技有限公司

TOP

掃一掃,關注我們!

首 頁| 公司介紹| 產(chǎn)品展示| 公司新聞| 技術文章| 聯(lián)系我們| 客戶留言

阿儀網(wǎng) 設計制作,未經(jīng)允許翻錄必究. 聯(lián)系人:錢經(jīng)理 聯(lián)系電話:18221656311 ICP備案號:滬ICP備11004148號-11 總訪問量:7661150 管理登錄

主營產(chǎn)品:ELISA試劑盒,人ELISA試劑盒,大鼠ELISA試劑盒,小鼠ELISA試劑盒,裸鼠ELISA試劑盒,倉鼠ELISA試劑盒,標準品,培養(yǎng)基和生物試劑等

14

阿儀網(wǎng)推薦收藏該企業(yè)網(wǎng)站

聯(lián)系方式

18221656311
13296034073

工作時間

(9:00-18:00)
粉嫩小又紧水又多A片| 人妻丰满熟妇无码区免费| 国产妇女馒头高清泬20P多| 亚洲国产成人精品无码区二本| 无码专区AAAAAA免费视频| 女人站着被舌头伺候| 男人自慰泄欲飞机GAY2022| 性高湖久久久久久久久| 中国老太卖婬HD播放| 少妇高潮喷水久久久久久久久| 香蕉久久一区二区不卡无毒影院| 欧美性猛交XXXX黑人猛交| av无码一区二区三区| 老汉的性生生活1一7| 穿着旗袍方便C英语老师| 国产精品一亚洲AV日韩AV欧| 欧美XBOX和PS大片| 樱花YY私人在线影院| 免费人成视频在线观看| 939W乳液78W实时更新| 亚洲第一综合天堂另类专| 色综合久久精品亚洲国产| 精品国产AV一区二区三区| 女人扒开屁股爽桶30分钟| 少妇人妻综合久久中文字幕| 国精品无码一区二区三区在线| 亚洲欧美一区二区三区在线| 女人睡着了摸她的G点会来高潮吗| 人妻少妇精品久久久久久| 丰满老熟妇好大BBBBB四P| 啊灬啊灬啊灬快喷水了视频| 阿公抱着我边摸边吃奶视频| 黑人猛挺进小莹的体内视频| 无限资源在线观看片中文在线| 久久精品无码一区二区三区免费| 奶头被教练摸得受不了| 怎么找附近的寂寞少妇| 欧美熟妇另类久久久久久多毛| 欧美牲交a欧美牲交| 中文字幕日韩欧美一区二区三区| 疯狂做受XXXX高潮欧美日本|